Affinity crosslinking agent for enzyme stabilization and immobilization

Technical Report · Mon Dec 31 23:00:00 EST 1973

OSTI ID:6595461

Guire, P E

This project was designed for the development of technology needed for the stabilization of enzymes in solution. Immobilized stabilized enzyme-cofactor couples are also expected to result from the research. The enzyme-ligand system chosen for specific emphasis was that of ferredoxin and hydrogenase which are potentially useful in the biophotolysis of water for generating hydrogen in a solar energy conversion system. A major objective is the efficient production of stabilized immobilized ferredoxin-hydrogenase couples functional in the transfer of electrons to hydrogen ions for the production of hydrogen gas from photosynthetically photolyzed water. Photoreactive derivatives of ferredoxin were prepared and their electron transfer activities investigated. Photochemical coupling to enzymes to form stabilized catalytically active enzyme-cofactor couples were also tested. The research is expected to demonstrate the utility of these stepwise crosslinking reagents for covalent stabilization and immobilization of individual enzymes, enzyme-cofactor couples, and enzyme couples with other enzymes, subcellular organelles and whole cells.

• Research Organization: Midwest Research Inst., Kansas City, MO (USA)
• OSTI ID: 6595461
• Report Number(s): PB-282423
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106* -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
14 SOLAR ENERGY
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCONVERSION
BIOPHOTOLYSIS
BIOSYNTHESIS
CHEMICAL REACTIONS
COENZYMES
CROSS-LINKING
DECOMPOSITION
ENZYMES
HYDROGEN PRODUCTION
HYDROGENASE
OXIDOREDUCTASES
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PHOTOSYNTHESIS
POLYMERIZATION
STABILIZATION
SYNTHESIS