Preparation of hydrogenase immobilized in polyvinyl alcohol film

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Appl. Biochem.; (United States)

OSTI ID:5228256

Yagi, T

Hydrogenase (hydrogen:ferricytochrome c/sub 3/ oxidoreductase, EC 1.12.2.1) from the particulate fraction of Desulfovibrio vulgaris, Miyazaki, was directly immobilized in polyvinyl alcohol film. The hydrogenase film thus prepared retained its activity during prolonged and repeated use. The half-life of the enzyme activity at pH 7.0 at 30/sup 0/C was 25-30 h, whereas that of the purified hydrogenase from the same bacterium was only 5 h under identical reaction conditions. The enzyme film was also active at pH 7.0 at 50/sup 0/C. The activation energy of the hydrogen-uptake reaction (with methylviologen as the electron acceptor) catalyzed by the hydrogenase film was estimated to be 53 kJ mol/sup -1/, a value identical to that of the purified hydrogenase. The immobilized hydrogenase film will be applied in the hydrogen production system by biophotolysis of water where it is expected to play a key role.

• Research Organization: Shizuoka Univ., Japan
• OSTI ID: 5228256
• Journal Information: J. Appl. Biochem.; (United States), Journal Name: J. Appl. Biochem.; (United States) Vol. 1:5-6; ISSN JABID
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106* -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BACTERIA
BIOCONVERSION
BIOPHOTOLYSIS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
FILMS
HYDROGEN COMPOUNDS
HYDROGEN PRODUCTION
HYDROGENASE
HYDROXY COMPOUNDS
IMMOBILIZED ENZYMES
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
POLYMERS
POLYVINYLS
PVA
WATER