Immobilized chloroplast-ferredoxin-hydrogenase system for the simultaneous photoproduction of hydrogen and oxygen
The chloroplast-ferredoxin-hydrogenase (CFH) system is capable of the simultaneous photoproduction of hydrogen and oxygen using water as the source of electrons. The immobilization of chloroplasts and hydrogenase, which has been the subject of several reports has been attempted in order to increase their storage and functional stability. However, immobilized chloroplasts and hydrogenase have never been shown to produce hydrogen and oxygen simultaneously. The simultaneous photoproduction of hydrogen and oxygen is a valuable guide in assessing the role of water as the primary substrate in biophotolysis. As has been shown previously, the stoichiometric ratio of hydrogen to oxygen is, in general, not equal to 2. By measuring the oxygen that is produced along with the hydrogen, it is possible to set an upper limit on the photoproduced hydrogen that is derive d from water. The study reported here describes for the first time the simultaneous photoproduction of hydrogen and oxygen by the CFH system immobilized in calcium alginate gel spheres. 15 references, 4 figures.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6925823
- Report Number(s):
- CONF-830567-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 13; ISSN BIBSB
- Country of Publication:
- United States
- Language:
- English
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080106* -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
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59 BASIC BIOLOGICAL SCIENCES
ALGINATES
ALKALINE EARTH METAL COMPOUNDS
BACTERIA
BIOCONVERSION
BIOPHOTOLYSIS
CALCIUM COMPOUNDS
CELL CONSTITUENTS
CHEMICAL REACTION YIELD
CHEMICAL REACTIONS
CHLOROPLASTS
CLOSTRIDIUM
COMPARATIVE EVALUATIONS
DECOMPOSITION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FERREDOXIN
FOOD
HYDROGEN PRODUCTION
HYDROGENASES
IMMOBILIZED CELLS
IMMOBILIZED ENZYMES
METALLOPROTEINS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PRODUCTION
PROTEINS
SPINACH
VEGETABLES
YIELDS