Limiting reactions in biosolar hydrogen and oxygen production
The first measurements of the steady-state turnover times of simultaneous photosynthetic hydrogen and oxygen production are reported. The system studied is the in vitro system composed of isolated spinach chloroplasts, ferredoxin, and hydrogenase (CFH). The simultaneous photoproduction of hydrogen and oxygen was measured by driving the CFH system into the steady state with repetitive single-turnover flash illumination. The turnover times for both the oxygen and hydrogen photoreactions are ca. 3 ms, which is about twice as fast as the analogous value for anaerobic photosynthesis in intact green algae. The number of apparent functional photosynthetic units for hydrogen and oxygen photoproduction is, however, only a few percent of the Emerson and Arnold value. It is concluded that this is the limiting aspect in the yields of hydrogen and oxygen photoproduction by the CFH system.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5354419
- Report Number(s):
- CONF-810554-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 11; ISSN BIBSB
- Country of Publication:
- United States
- Language:
- English
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080106 -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
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140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLOROPLASTS
ELEMENTS
ENZYMES
FERREDOXIN
HYDROGEN PRODUCTION
HYDROGENASES
METALLOPROTEINS
NONMETALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PRODUCTION
PROTEINS
SYNTHESIS
YIELDS