Comparative dual tracer studies of. beta. -methyl-(1-C-14)heptadecanoic acid (BMHDA) and 15-p-(I-131)-iodophenyl-. beta. -methyl pentadecanoic acid (BMPDA) in hypertensive rats

Conference · Sat Dec 31 23:00:00 EST 1983 · J. Nucl. Med.; (United States)

OSTI ID:6590984

Yamamoto, K; Som, P; Brill, A B; Yonekura, Y; Goodman, M M; Knapp, Jr, F F; Elmaleh, D R; Strauss, H W

C-11 labeled BMHDA, a branched chain fatty acid, has been reported to be a potential myocardial metabolic tracer for positron emission tomography (PET). The authors have studied the distribution of C-14 BMHDA and I-131 BMPDA in hypertensive rat hearts, using quantitative dual isotope autoradiographic (ARG) techniques. Dahl stain rats (blood pressure: 214+-8 mmHg) were injected with 10 ..mu..Ci of C-14 BMHDA and 170 ..mu..Ci of I-131 BMPDA. Just after sacrifice, hearts and lungs were removed and processed for ARG. The tissue sections (20 ..mu..m thick) were placed on x-ray film for 1 day to reveal the distribution of I-131. The 2nd exposure for C-14 was performed 3 months later to permit the decay of I-131. Selected pairs of ARG images were digitized and quantitated using a videodensitometric system. C-14 BMHDA showed very heterogeneous distribution in the hypertensive myocardium. Decreased uptake was seen in the endocardial region. I-131 BMPDA showed the same uneven distribution as C-14 BMHDA. The correlation coefficients of these two tracers distribution calculated from paired quantitative images were excellent (0.91-0.96). The data suggest that I-123 labeled BMPDA could be used as a biochemical market to measure fatty acid derangement for single photon emission tomography, and may have wider application than C-11 BMHDA which is limited due to availability of PET devices.

• Research Organization: Brookhaven National Lab., Upton, NY
• DOE Contract Number: AC02-76CH00016; W-7405-ENG-26
• OSTI ID: 6590984
• Report Number(s): CONF-840619-
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BNL
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CAT SCANNING
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DRUGS
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
EMISSION COMPUTED TOMOGRAPHY
EVALUATION
HEART
HYPERTENSION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
NATIONAL ORGANIZATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POSITRON COMPUTED TOMOGRAPHY
RADIOISOTOPE SCANNING
RADIOPHARMACEUTICALS
RATS
RESPIRATORY SYSTEM
RODENTS
SCINTISCANNING
SYMPTOMS
TISSUE DISTRIBUTION
TOMOGRAPHY
TRACER TECHNIQUES
UPTAKE
US AEC
US DOE
US ERDA
US ORGANIZATIONS
VASCULAR DISEASES
VERTEBRATES