Correlation of pancreatic secretion and plasma cholecystokinin concentrations after various stimuli in dogs
The objectives of this study were to develop a sensitive, specific, and reliable radioimmunoassay for plasma cholecystokinin and to use the cholecystokinin radioimmunoassay (CCK RIA) to determine the role of CCK in stimulating exocrine pancreatic secretion. Three rabbits were immunized with synthetic sulfated CCK-10 conjugated to keyholed limpet hemocyanin. Using /sup 125/I-CCK-39 (iodo-gen) as tracer, it was found that antiserum R016 (titer = 1:256,000) recognized short and long forms of CCK and had negligible cross-reactivity with gastrin and other peptides. Plasma CCK levels and pancreatic secretion in fasted dogs (N = 6) were measured in response to a meat meal, intestinal perfusion with saline, graded amounts of sodium oleate (+/-atropine), a mixture of tryptophan/phenylalanine (Trp/Phe), or HCl, and i.v. infusion of graded doses of CCK-8 or CCK-39.
- Research Organization:
- Missouri Univ., Columbia (USA)
- OSTI ID:
- 6589915
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GASTROINTESTINAL TRACT
GLANDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PERFUSED ORGANS
POLYPEPTIDES
PROTEINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SECRETION
STIMULI
TRACER TECHNIQUES
VERTEBRATES