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Carbachol regulates cholecystokinin receptor on pancreatic acinar cells

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:6469541
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The authors have examined the effect of carbamylcholine on the binding of cholecystokinin (CCK) to dispersed acini from rat pancreas. The CCK receptor on pancreatic acini possesses two classes of binding sites. Simultaneous addition of carbamylcholine inhibited binding of CCK binding sites. Atropine prevented the inhibitory effect of carbamylcholine, whereas calcium ionophore A23187 did not alter binding of CCK. 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibited binding of CCK in the same manner as carbamylcholine. Inhibition by carbamylcholine was reversible and the recovery was time dependent. By contrast, inhibition of binding of CCK by TPA did not reverse after a 60-min incubation without the agent. These findings, at least in part, account for the inhibition of the CCK-induced stimulation of amylase secretion by carbamylcholine. The action of TPA on binding of CCK suggests the possible involvement of the activation of protein kinase C in the inhibition of binding.

Research Organization:
Kyoto Univ. School of Medicine, Japan
OSTI ID:
6469541
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:1; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM COMPOUNDS
CARCINOGENS
CHOLINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ESTERS
GLANDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININS
LIPOTROPIC FACTORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PARASYMPATHOLYTICS
PEPTIDES
PHORBOL ESTERS
POLYPEPTIDES
PROMOTERS
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RADIORECEPTOR ASSAY
TRACER TECHNIQUES
TUMOR PROMOTERS