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Cholinergic stimulation of pancreatic amylase release and muscarinic receptors: effect of ionophore A23187

Journal Article · · Life Sci.; (United States)
;

Dispersed rat pancreatic acini were incubated in 0.5 mM calcium medium with increasing concentrations of carbamylcholine, with or without the ionophore A23187 (10/sup -6/M). Addition of the ionophore reduced maximal amylase release, increased the maximal effective concentration of carbamylcholine and dramatically impaired the agonist's capacity to induce enzyme secretion at low concentration. The ionophore also abolished the inhibition of secretion observed at high carbamylcholine concentrations. These effects of the ionophore on the cholinergic secretory response cannot be explained by interaction at the muscarinic receptor since neither the Bmax, the affinity of the receptor for the (/sup 3/H)QNB nor the binding of carbamylcholine were affected by the ionophore. It is suggested that for the conditions studied, the ionophore can interact with the secretory process at one or several points ulterior to the initial recognition site of carbamylcholine on its receptor. 30 references, 3 figures.

Research Organization:
Universite de Sherbrooke, Quebec
OSTI ID:
5411334
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:3; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALCOHOLS
ALKALINE EARTH METALS
AMINES
AMMONIUM COMPOUNDS
AMYLASE
ANIMALS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CHEMICAL BONDS
CHOLINE
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
METALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
QUATERNARY COMPOUNDS
RATS
RECEPTORS
RODENTS
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES