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Influence of major structural features of cellulose on rate of enzymatic hydrolysis

Journal Article · · Biotechnol. Bioeng.; (United States)

An attempt was made to determine the effect of two structural features of cellulose on its enzymatic hydrolysis: its crystallinity and its specific surface area. Solka Floc SW40 was pretreated in various ways prior to hydrolysis: ball milling, gamma radiation, pyrolysis, treatment by sodium hydroxide, treatment by CMCS, and treatment by sulfuric acid. Microcrystalline cellulose (Sigmacell 50) was also employed in the test. The culture filtrate of Trichoderma reesei QM9414 was the source of the enzyme. It was observed that, independent of the methods of treatment, the rate of hydrolysis would tend to increase with an increase in specific surface area and with a decrease in crystallinity index.

Research Organization:
Kans State Univ, Manhattan, USA
OSTI ID:
6584367
Journal Information:
Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:2; ISSN BIBIA
Country of Publication:
United States
Language:
English