Effect of. beta. -glucosidase on the enzymatic hydrolysis of cellulose. [Botryodiplodia theobromae, Trichoderma viride]

Technical Report · Mon Dec 01 04:00:00 EST 1975

DOI:https://doi.org/10.2172/7122570· OSTI ID:7122570

Yamanaka, Y; Wilke, C R

A fourteen liter fermentor was used to produce ..beta..-glucosidase with the fungus Botryodiplodia theobromae with a reported medium, but better results were obtained with a medium used for the production of cellulase by Trichoderma viride. The optimum conditions for the batch fermentation were pH 5.0 and 30/sup 0/C, and the ..beta..-glucosidase activity after seven days of fermentation was 1.0 mg/ml, which was a tentatively set target value in the present work. The continuous production of the enzyme was also investigated, and the results showed that a two-stage fermentation process (fungus growth on a glucose medium, followed by transfer to a cellulose medium for enzyme induction) worked as well as the two-stage production of cellulase from Trichoderma viride. The effect of ..beta..-glucosidase is much more pronounced when the accessibility of the substrate is high, as with ball-milled Solka Floc, and when the cellulase activity of the original cellulase preparation is low. The hydrolysis of newsprint, in which cellulose accessibility is low, by solutions with high cellulase activity is improved by use of a mixed enzyme system to a much lesser degree than for Solka Floc. Mxitures containing 15 to 30 percent of Botryodiplodia theobromae culture filtrate mixed with Trichoderma viride filtrate produce a 10 to 20 percent increase in the total conversion of cellulose and a 20 to 40 percent increase in the glucose productivity in hydrolysis of newsprint.

• Research Organization: California Univ., Berkeley (USA). Lawrence Berkeley Lab.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 7122570
• Report Number(s): LBL-4413
• Country of Publication: United States
• Language: English

Related Subjects

500200* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
BIOCONVERSION
BIOMASS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENERGY SOURCES
ENZYMES
FERMENTATION
FUNGI
HYDROLYSIS
LYSIS
ORGANIC COMPOUNDS
PH VALUE
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS