Decomposition of lignin and cellobiose in relation to the enzymatic hydrolysis of cellulose

Conference · Tue Feb 01 04:00:00 EST 1977

OSTI ID:7284136

Yamanaka, Y; Carroad, P A; Riaz, M; Wilke, C R

Studies are reported on the use of fungal ..beta..-glucosidase in conjunction with Trichoderma viride cellulase and the search for an effective enzyme system for lignin degradation. ..beta..-glucosidase is of potential benefit in cellulose hydrolysis by catalyzing the hydrolysis of cellobiose to glucose thereby reducing product inhibition and producing a higher glucose yield. Removal of lignin from cellulosic material makes the cellulose more accessible to hydrolyzing enzymes. Hydrolysis studies on Solka Floc and newsprint were conducted with T. viride filtrates containing various proportions of B. theobromae filtrates. Significant improvement in hydrolysis rate particularly in glucose content was obtained by thus enriching the ..beta..-glucosidase content of the cellulase. In the search for a lignin degrading enzyme, major emphasis was given to the fungus Polyporous versicolor. Significant o-diphenol oxidoreductase (catecholase) activity was found in the culture filtrates. Preliminary observations of a surface culture of the fungus in a composting mode suggest that delignification may be obtained in this manner. Work is continuing on this.

• Research Organization: California Univ., Berkeley (USA). Lawrence Berkeley Lab.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 7284136
• Report Number(s): LBL-5960; CONF-761166-1
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOMASS
CARBOHYDRATES
CATALYSIS
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENERGY SOURCES
ENZYMES
FUNGI
GLUCOSE
HEXOSES
HYDROLYSIS
KINETICS
LIGNIN
LYSIS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
POLYSACCHARIDES
REACTION KINETICS
RENEWABLE ENERGY SOURCES
RESEARCH PROGRAMS
SACCHARIDES
SOLVOLYSIS