Genetic and biochemical analysis of the CYC7-H3 mutation that causes overproduction of yeast ISO-2-cytochrome C
Technical Report
·
OSTI ID:6583835
The CYC7-H3 mutation causes a 20-fold cis-dominant overproduction of iso-2-cytochrome c in the yeast Saccharomyces cerevisiae. The CYC7-H3 mutation is a deletion of approximately 5 kilobase-pairs with one breakpoint located between 140 and 290 base-pairs on the 5' side of the AUG initiation codon of CYC7 and the other breakpoint located near a reiterated sequence. The deletion results in the appearance of two abundant RNA transcripts of length 840 and 680 nucleotides and apparently alters the structure, but not the size, of a 1750 nucleotide transcript. The results indicate that overproduction of iso-2-cytochrome c in the CYC7-H3 mutant is due to the fusion of a new regulatory signal to the CYC7 gene by a deletion. The fused regulatory signal may be affecting RNA processing, transcriptional initiation, or both, and may be related to the reiterated sequence fused to the CYC7 gene. The CYC7-H3 deletion encompasses two adjacent genes, RAD23 and ANP1, which control a total of four recessive phenotypes. The RAD23 gene is located centromere-proximal to CYC7, controls UV sensitivity and apparently functions in excision-repair. The ANP1-RAD23-CYC7 gene cluster discovered and characterized in this research displays striking similarity to the gene cluster containing the iso-1-cytochrome c gene, CYC1-OSM1-RAD7. The circularly permuted gene orders of these gene clusters and the similarity in their encoded functions suggests thay may be related by an ancient transpositional event. 116 references, 19 figures, 6 tables.
- Research Organization:
- Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6583835
- Report Number(s):
- DOE/EV/03490-2041; ON: DE84016262
- Country of Publication:
- United States
- Language:
- English
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