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An extensive deletion causing overproduction of yeast iso-2-cytochrome c

Journal Article · · Cell; (United States)
CYC7-H3 is a cis-dominant regulatory mutation that causes a 20-fold overproduction of yeast iso-2-cytochrome c. The CYC7-H3 mutation is an approximately 5 kb deletion with one breakpoint located in the 5' noncoding region of the CYC7 gene, approximately 200 base from the ATG initiation codon. The deletion apparently fuses a new regulatory region to the structural portion of the CYC7 locus. The CYC7-H3 deletion encompasses the RAD23 locus, which controls UV sensitivity and the ANP1 locus, which controls osmotic sensitivity. The gene cluster CYC7-RAD23-ANP1 displays striking similarity to the gene cluster CYC1-OSM1-RAD7, which controls, respectively, iso-1-cytochrome c, osmotic sensitivity and UV sensitivity. We suggest that these gene clusters are related by an ancient transpositional event.
Research Organization:
Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, New York
OSTI ID:
5547953
Journal Information:
Cell; (United States), Journal Name: Cell; (United States) Vol. 25:2; ISSN CELLB
Country of Publication:
United States
Language:
English