Environmental stress-mediated changes in transcriptional and translational regulation of protein synthesis in crop plants: Progress report, August 1985--August 1988
Research during the current grant period included isolation and characterization of a number of HS-related cDNAs and genomic clones; this activity included the sequencing of two genomic clones of the 13-member pCE53 family of genes, one of the pFS2033 family, one of the hsp70 family, partial sequences of three genomic clones of the pEV set of clones, and corresponding cDNAs to these genomic clones. Additional clones related to the hsp83 family were isolated from both soybean and Arabidopsis and partially characterized. Considerable effort has gone into attempts to isolate clones to the hsp90 and 110 families; while promising results were obtained, no definitive data are available on clone identity. This information provides a data base of HS genes that will be exceedingly useful in planned experiments as well as important information on the complexity and relationships of hsp families in plants. HS promoter cassettes were developed using the Gmhsp17.5-E clone (pFS2019cDNA); the promoter of this gene was extensively characterized by Czarnecka and Gurley. This promoter cassette was used to analyze the expression of a number of genes in transient assays using isolated protoplasts, with ..beta..-1,3 glucanase (GUS) proving to be a very useful reporter gene. Perfect fusions proved to provide higher levels of expression of GUS than the array of non-perfect fusions tested. This system provides a very efficient and useful assay for different promoter/gene constructs prior to using them in transformation/transgenic plant analyses. The utility of the cassette was demonstrated by studies on expression of the isopentenyltransferase gene from T-DNA in transgenic tobacco plants.
- Research Organization:
- Georgia Univ., Athens (USA)
- DOE Contract Number:
- FG09-86ER13602
- OSTI ID:
- 6581985
- Report Number(s):
- DOE/ER/13602-3; ON: DE89006477
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ARABIDOPSIS
ARSENIC COMPOUNDS
ARSENIDES
BIOLOGICAL ADAPTATION
CADMIUM
CLONING
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
ELEMENTS
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
NICOTIANA
ORGANIC COMPOUNDS
PLANTS
PNICTIDES
PROGRESS REPORT
PROTEINS
TEMPERATURE DEPENDENCE