Environmental stress-mediated changes in transcriptional and translational regulation of protein synthesis in crop plants

Technical Report · Sat Jun 01 00:00:00 EDT 1991

OSTI ID:5736769

Key, J L; Nagao, R T

The major research activities accomplished during the renewal period focused on defining regulatory mechanisms operative in the heat shock (HS) response and assessing the mechanism of HS-induced thermotolerance in Arabidopsis. HS gene regulation was studied by transcriptional run-off assays, and self regulation of heat shock protein (hsp) levels and synthesis was studied through use of amino acid analogs and protein synthesis inhibitors. Also studied were subcellular localization of hsps during HS and HS recovery, characterization of membrane bound hsps and their effects on membrane proton transport, and the influence of over- or underexpression of HS mRNAs and hsps plant phenotype using transgenic plants. In addition, hsp70 and hsp83 cDNAs/genes are being characterized and their expression patterns will be evaluated. 10 refs. (MHB)

• Research Organization: Georgia Univ., Athens, GA (USA)
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG09-86ER13602
• OSTI ID: 5736769
• Report Number(s): DOE/ER/13602-4; ON: DE91015240
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ARABIDOPSIS
BARYONS
BIOLOGICAL ADAPTATION
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
DISTRIBUTION
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
ELEMENTARY PARTICLES
ENZYME INHIBITORS
FERMIONS
GENE REGULATION
HADRONS
HYBRIDIZATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PLANTS
PROGRESS REPORT
PROTEINS
PROTONS
RNA
SUBCELLULAR DISTRIBUTION
SYNTHESIS
TEMPERATURE EFFECTS
TOLERANCE
TRANSCRIPTION