Autoradiographic localization of adenosine receptors in rat brain using (/sup 3/H)cyclohexyladenosine

Journal Article · Wed Sep 01 00:00:00 EDT 1982 · J. Neurosci.; (United States)

OSTI ID:6580300

Goodman, R R; Synder, S H

Adenosine (A1) receptor binding sites have been localized in rat brain by an in vitro light microscopic autoradiographic method. The binding of (/sup 3/H)N6-cyclohexyladenosine to slide-mounted rat brain tissue sections has the characteristics of A1 receptors. It is saturable with high affinity and has appropriate pharmacology and stereospecificity. The highest densities of adenosine receptors occur in the molecular layer of the cerebellum, the molecular and polymorphic layers of the hippocampus and dentate gyrus, the medial geniculate body, certain thalamic nuclei, and the lateral septum. High densities also are observed in certain layers of the cerebral cortex, the piriform cortex, the caudate-putamen, the nucleus accumbens, and the granule cell layer of the cerebellum. Most white matter areas, as well as certain gray matter areas, such as the hypothalamus, have negligible receptor concentrations. These localizations suggest possible central nervous system sites of action of adenosine.

• Research Organization: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland
• OSTI ID: 6580300
• Journal Information: J. Neurosci.; (United States), Journal Name: J. Neurosci.; (United States) Vol. 2:9; ISSN JNRSD
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMALS
AUTORADIOGRAPHY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CEREBRAL CORTEX
CEREBRUM
HYPOTHALAMUS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RATS
RECEPTORS
RIBOSIDES
RODENTS
SPECIFICITY
THALAMUS
TRITIUM COMPOUNDS
VERTEBRATES