Autoradiographic localization of adenosine uptake sites in rat brain using (/sup 3/H)nitrobenzylthioinosine

Journal Article · Thu Jan 31 23:00:00 EST 1985 · J. Neurosci.; (United States)

OSTI ID:5480746

Bisserbe, J C; Patel, J; Marangos, P J

The adenosine uptake site has been localized in rat brain by an in vitro light microscopic autoradiographic method, using (/sup 3/H)nitrobenzylthioinosine ((/sup 3/H)NBI) as the probe. The binding characteristics of (/sup 3/H)NBI on slide-mounted sections are comparable to those seen in studies performed on brain homogenates. A very high density of uptake sites occurs in the nucleus tractus solitarius, in the superficial layer of the superior colliculus, in several thalamic nuclei, and also in geniculate body nuclei. A high density of sites are also observed in the nucleus accumbens, the caudate putamen, the dorsal tegmentum area, the substantia nigra, and the central gray. The localization of the adenosine uptake site in brain may provide information on the functional activity of the site and suggests the involvement of the adenosine system in the central regulation of cardiovascular function.

• Research Organization: National Institute of Mental Health, Bethesda, MD
• OSTI ID: 5480746
• Journal Information: J. Neurosci.; (United States), Journal Name: J. Neurosci.; (United States) Vol. 5:2; ISSN JNRSD
• Country of Publication: United States
• Language: English

Related Subjects

550801* -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMALS
AROMATICS
AUTORADIOGRAPHY
AZAARENES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
HETEROCYCLIC COMPOUNDS
INOSINE
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PURINES
RATS
RECEPTORS
RIBOSIDES
RODENTS
THALAMUS
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES