Ionizing radiation induces early, sustained increases in collagen biosynthesis: a 48-week study in mouse skin and skin fibroblast cultures

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577485· OSTI ID:6579840

Panizzon, R G; Hanson, W R; Schwartz, D E; Malkinson, F D

Groups of 10 CF1 female mice, irradiated to the thorax with a dual-head /sup 137/Cs gamma-RAY source, received single doses of 0, 5, 10, 15, or 25 Gy. One to forty-eight weeks later collagen synthesis was measured in minced skin specimens incubated in medium containing (/sup 3/H)proline and then assayed for radioactive hydroxyproline. A progressive, generally dose-dependent increase in collagen biosynthesis, up to 50% above control sites, was found 1, 4, and 12 weeks after radiation exposure. These changes showed further small fluctuations at 12-36 weeks, increasing again at the 48-week interval. At the same times throughout the study fibroblasts were cultured from skin explants. Following the second subculture, these cells were also incubated in medium containing (/sup 3/H)proline, and collagen synthesis was again determined by (/sup 3/H)hydroxyproline assay. At all radiation dose levels studied, collagen production increased threefold by 12 weeks postradiation and remained elevated for the 48-week duration of the study. In vitro radiation dose response differences were not observed.

• Research Organization: Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL (USA)
• OSTI ID: 6579840
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 116:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BODY AREAS
CARBOXYLIC ACIDS
CESIUM 137
CESIUM ISOTOPES
CHEST
COLLAGEN
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
FIBROSIS
GAMMA RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SCLEROPROTEINS
SKIN
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
YEARS LIVING RADIOISOTOPES