Collagen Content of Lungs of Mice after X-Ray Irradiation

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Radiation Research

DOI:https://doi.org/10.2307/3574287· OSTI ID:4015616

Law, Marilyn P.; Hornsey, Shirley; Field, Stanley B.

To investigate the development of late radiation fibrosis in lungs large single doses of x rays were given to the hemithoraxes of mice. Hydroxyproline, an amino acid specific to collagen, was estimated in both irradiated and shielded lungs up to 48 weeks after exposure. There was a dose dependent atrophy of the irradiated lung and a compensatory hypertrophy of the shielded lung. The hydroxyproline per gram of dry irradiated lung increased between 24 and 36 weeks after 2000 to 4000 rad with no further change up to 48 weeks. Both total hydroxyproline content and dry weight of irradiated lungs decreased but after 24 weeks the histological appearance was that of collagen deposition after edema and hyperplasia. It is concluded that collagen deposition occurs at a late stage in the reorganization after radiation injury to mouse lung and is therefore probably not the cause of 80 to 180 day death after exposure of the whole thorax.

• Research Organization: Hammersmith Hospital, London
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-026283
• OSTI ID: 4015616
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 65; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: United States
• Language: English

Related Subjects

*FIBROSIS-- RADIOINDUCTION
*LUNGS-- DELAYED RADIATION EFFECTS
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
CHEST
COLLAGEN
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
PARTIAL BODY IRRADIATION
QUANTITY RATIO
TIME DEPENDENCE
X RADIATION