Effects of chronic exposure to ozone on collagen in rat lung

Journal Article · Tue Mar 15 04:00:00 EST 1988 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(88)90184-6· OSTI ID:6936275

Wright, E S; Kehrer, J P; White, D M; Smiler, K L

Pulmonary fibrosis is a consequence of severe injury from some toxic agents including high doses of ozone. It is not known, however, whether chronic exposure to low doses of ozone, such as those encountered in polluted ambient atmospheres, could also result in abnormal accumulations of lung collagen. Rats were exposed to ozone for 20 hr per day, 7 days per week for 3, 6, 12, and 18 months at concentrations of 0.12, 0.25, or 0.50 ppm. Controls were exposed under identical conditions to purified air. Upon removal from the chambers, rats were euthanized and lung tissue slices incubated with (14C)proline. The incorporation of 14C into hydroxyproline and the total hydroxyproline content of lung tissue were measured as estimates of lung collagen synthesis and content, respectively. The formation of labeled hydroxyproline tended to decrease significantly with time in controls and at the three ozone doses. There were, however, no significant dose-related changes at any of the time points tested. Total lung hydroxyproline increased with age in all groups, but no dose-related changes were detected at any time point. It was concluded that chronic exposure of rats to ozone at concentrations which approximate ambient urban concentrations did not affect normal age-related changes in either synthesis or accumulation of lung collagen.

• Research Organization: General Motors Research Labs, Warren, MI (USA)
• OSTI ID: 6936275
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 92:3; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHRONIC EXPOSURE
COLLAGEN
DOSE-RESPONSE RELATIONSHIPS
FIBROSIS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LUNGS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OZONE
PATHOLOGICAL CHANGES
POLLUTION
PROTEINS
PYRROLES
PYRROLIDINES
RATS
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SYNTHESIS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES