Simple, rapid /sup 125/I-labeled cyclosporine double antibody/polyethylene glycol radioimmunoassay used in a pediatric cardiac transplant program

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Ther. Drug Monit.; (United States)

DOI:https://doi.org/10.1097/00007691-198612000-00017· OSTI ID:6575545

Berk, L S; Webb, G; Imperio, N C; Nehlsen-Cannarella, S L; Eby, W C

We modified the Sandoz cyclosporine radioimmunoassay because of our need for frequent clinical monitoring of cyclosporine drug levels in allo- and xenograft pediatric cardiac transplant patients. With application of a commercially available (/sup 125/I)cyclosporine label in place of (/sup 3/H)cyclosporine and a second antibody/polyethylene glycol (PEG) method of separation in place of charcoal separation, we simplified and enhanced the speed and precision of assay performance. Studies of 140 whole blood samples comparing this new method to the (/sup 3/H)cyclosporine radioimmunoassay (RIA) method of Berk and colleagues yielded a coefficient of correlation of 0.96 (p less than 0.00001) with means of 626 and 667 ng/ml for (/sup 3/H)RIA and (/sup 125/I)RIA, respectively, and a regression equation of y = 28 + 1.02x. The major advantages are that total assay time is reduced to approximately 1 h; (/sup 125/I)cyclosporine label is used, avoiding the problems associated with liquid scintillation counting; and precision is enhanced by separating bound and free fractions with second antibody/PEG. These modifications should provide for greater ease of assay performance and improved clinical utility of cyclosporine monitoring not only in the pediatric but also in the adult transplant patient.

• Research Organization: Loma Linda Univ. Medical Center, CA
• OSTI ID: 6575545
• Journal Information: Ther. Drug Monit.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
HEART
TRANSPLANTS
IMMUNOSUPPRESSIVE DRUGS
RADIOIMMUNOASSAY
CHILDREN
GRAFT-HOST REACTION
IODINE 125
PATIENTS
POLYETHYLENE GLYCOLS
QUALITY CONTROL
TRITIUM COMPOUNDS
AGE GROUPS
ALCOHOLS
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DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOLS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
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RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550600 - Medicine