Amplifications and deletions in clinical ovarian cancer detected by Comparative Genomic Hybridization (CGH)

Conference · Thu Dec 31 23:00:00 EST 1992 · Cytometry (Baltimore); (United States)

OSTI ID:6572941

Sakunaga, H; Sakamoto, M; Kallioniemi, A; Kallioniemi, O; Sudar, D; Pinkel, D; Gray, I W ^[1]; Yang-Feng, T ^[2]

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1. Univ. of California, San Francisco (United States)
2. Yale Univ., New Haven, CT (United States)

CGH is a new powerful method for surveying the whole genome for DNA sequence copy number changes in a single hybridization. The method is based on the competition between biotinylated total tumor DNA and a digoxigenin-labeled normal genomic reference DNA during hybridization to normal metaphase chromosomes. After immunofluorescent staining with avidin-FITC and antidigoxigenin Rhodamine, variation of DNA sequence copy numbers in the tumor are detected as variations in the ratios of green and red fluorescence along each chromosome. The authors applied CGH analysis to DNA extracted from surgically removed ovarian cancer specimens (27 cases). Seven amplified regions were identified by CGH analysis. Three loci, 1p32-p34 (most likely, MYCL), 8q23-q24 (MYC), 12q12 (KRAS2), were known to be amplified in solid tumors and four other loci (3q26, 6p22, 9q31-q33, 17q22) were previously unknown to be amplified. Many regions indicating physical deletions were also identified by the analysis. Chromosomal regions showing frequent deletion were 1p, 3p, 17p, 17q, 19p, 19q and Xp. There were also significant similarities of the regions with amplifications and deletions between bilateral ovarian tumors or among several different tumors form the same ovarian cancer cases, suggesting that the genetic changes observed might be relatively early events during the progression of ovarian cancer.

• OSTI ID: 6572941
• Report Number(s): CONF-9303114--
• Journal Information: Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 6; ISSN CYTODQ; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AZOLES
BIOTIN
BODY
CARBOXYLIC ACIDS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DETECTION
DISEASES
DNA HYBRIDIZATION
FEMALE GENITALS
FLUORESCENCE
GENE AMPLIFICATION
GONADS
HETEROCHROMOSOMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HUMAN CHROMOSOME 17
HUMAN CHROMOSOME 19
HUMAN CHROMOSOME 3
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
HYBRIDIZATION
IMIDAZOLES
LABELLING
LUMINESCENCE
MUTATIONS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OVARIES
VITAMIN B GROUP
VITAMINS
X CHROMOSOME