Increased copy number at 20q13 and 17q22-q24 in breast cancer: Strategies for identification of two new putative oncogene loci
- Tampere Univ. Hospital (Finland); and others
Our studies using comparative genomic hybridization (CGH) have indicated that two chromosomal regions, 17q22-q24 and 20q13, are often present at an increased copy number in breast cancer. Here, we sought to study these putative new oncogene loci in more detail by identifying the critical overlapping regions of amplification in several tumors and cell lines and by studying involvement of cloned genes in these regions. Fluorescence in situ hybridization (FISH) and a set of 150 anonymous cosmid clones and gene-specific P1 clones mapped along chromosome 20 were used to assess copy number in 14 breast cancer cell lines and 36 primary tumors. Based on the high-level copy number increases (3- to 10-fold) found in 5 cell lines and 3 primaries, the critical region was narrowed down to {approximately}1.5 Mb at 20q13.2 defined by FLpter values 0.81-0.84. All previously known genes were excluded as targets for amplification based either on their chromosomal location or lack of high-level amplification. Similar studies of the 17q22-q24 region have identified at least three separate sites of increased copy number with FLpter values 0.62, 0.76, and 0.89 in breast cancer cell lines. The region at 0.76 appeared to be highly amplified in at least two breast cancer cell lines and is now being evaluated in primary tumors. In conclusion, our strategies for the identification of putative new oncogene loci based on the genome-scale screening for amplification by CGH followed by detailed amplicon mapping by FISH have lead to the identification of two small chromosomal regions that are likely to harbor genes important for breast cancer progression.
- OSTI ID:
- 133343
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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