Comparative genomic hybridization reveals many new loci containing amplified genes in breast cancer

Conference · Fri Jan 01 04:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6558362

Kallioniemi, O P; Kallioniemi, A H; Rutovitz, D; Sudar, D; Chen, L C; Smith, H S; Gray, J W; Pinkel, D; Waldman, F M ^[1]

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1. Univ. of California, San Francisco (United States) GBCRI, San Francisco, CA (United States) MRC Genetics Unit, Edinburgh (United Kingdom)

The authors have developed a powerful new technique, comparative genomic hybridization (CGH), for molecular cytogenetic analysis of solid tumors. In CGH, differentially labeled total tumor DNA and normal reference DNA are allowed to compete for their binding sites in a normal metaphase spread. After immunofluorescent staining, the relative copy numbers of all regions in the tumor genome can be quantitiated with an image analysis system by measuring the intensity ratios of the two fluorochromes along the length of each chromosome. They used CGH to study gene amplification and other chromosomal changes in 16 breast cancer cell lines and 20 primary tumors. The loci undergoing amplification were highly variable from one sample to another and over 20 distinct sites were identified. Some sites correspond to locations of known oncogenes (e.g. erbb2 at 17q12 and myc at 8q24) whereas most of them (e.g. 6p22, 11p15, 17q22) are not previously known to contain amplified oncogenes. Frequent changes affecting larger chromosomal regions, such as duplications of 1q and deletions of 1p32-35, have also been found. CGH can dramatically facilitate identification of commonly altered chromosomal loci in cancer.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6558362
• Report Number(s): CONF-9303114--; CNN: CA 45919; CA 47537
• Conference Information: Journal Name: Cytometry (Baltimore); (United States) Journal Volume: 6
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOASSAY
BODY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DETECTION
DISEASES
DNA HYBRIDIZATION
FLUORESCENCE
GENE AMPLIFICATION
GENES
GLANDS
HUMAN CHROMOSOME 17
HUMAN CHROMOSOMES
HYBRIDIZATION
IMMUNOASSAY
LUMINESCENCE
MAMMARY GLANDS
MUTATIONS
NEOPLASMS
ONCOGENES
ORGANS