Tissue enzyme changes in parabiotic rats with subcutaneous lymphoma

Technical Report · Sat Jan 01 04:00:00 EST 1977

DOI:https://doi.org/10.2172/6569071· OSTI ID:6569071

Herzfeld, A; Greengard, O; Warren, S

A solid lymphoma was implanted into normal rats and into one partner of parabiotic pairs. Appreciable decreases in hepatic ornithine aminotransferase and arginase occurred about a week earlier (4 to 6 days after implantation) in single than in parabiotic hosts. By 18 to 21 days significant decreases in both enzymes were also apparent in the host partner. The hepatic thymidine kinase showed a 5-fold elevation in single hosts 4 days after implantation; by 14 days its levels were about 200 times above normal and had also risen in the parabiotic hosts (20-fold) and the host partners (4-fold). Implantation of fibrosarcoma caused qualitatively similar but generally less pronounced changes in the above 3 enzymes in livers of single hosts, parabiotic hosts, and host partners. The splenic thymidine kinase, 14 days after implantation, was increased from control levels of about 3 units/g to 50.6, 44.8, and 13.5 units/g in single hosts, parabiotic hosts, and host partners, respectively. At later stages, 17 to 20 days after implantation of the lymphoma, appreciable amounts of thymidine kinase appeared in the plasma: the units of activity per ml were 6.2 in single hosts, 0.79 in parabolic hosts, and 0.55 in host partners (control <0.05). The observations on the hepatic and splenic enzyme changes in parabiotic rats suggest that effects of neoplasms on distant host tissues are mediated by humoral factors. The less pronounced responses in parabiotic than in single hosts indicate that the tumor-free partner affords some protection against these systemic effects.

• Research Organization: New England Deaconess Hospital, Boston, MA (USA). Cancer Research Inst.; Harvard Medical School, Boston, MA (USA). Dept. of Biological Chemistry
• Sponsoring Organization: USDOE
• DOE Contract Number: AS02-76EV03017
• OSTI ID: 6569071
• Report Number(s): DOE/EV/03017-T1
• Country of Publication: United States
• Language: English

Related Subjects

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550900 -- Pathology
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59 BASIC BIOLOGICAL SCIENCES
AMIDASES
AMINOTRANSFERASES
ANIMALS
ARGINASE
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DISEASES
ENZYME ACTIVITY
ENZYMES
HYDROLASES
IMPLANTS
LYMPHOMAS
MAMMALS
MATERIALS
MOSAICISM
NEOPLASMS
NITROGEN TRANSFERASES
NON-PEPTIDE C-N HYDROLASES
PARABIOSIS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
QUANTITY RATIO
RATS
RODENTS
TRANSFERASES
VERTEBRATES