Tissue enzyme changes in parabiotic rats with subcutaneous lymphoma or fibrosarcoma. [Ornithine aminotransferase, orginase, thymidine kinase]

Journal Article · Sat Apr 01 04:00:00 EST 1978 · J. Natl. Cancer Inst.; (United States)

OSTI ID:5143555

Herzfeld, A; Greengard, O; Warren, S

A solid lymphoma implanted into normal inbred Kx rats and one partner of parabiotic pairs caused appreciable decreases in hepatic ornithine aminotransferase and arginase about a week earlier (4 to 6 days after implantation) in single hosts than in parabiotic hosts. By 18 to 21 days significant decreases in both enzymes were apparent in the host partner also. The hepatic thymidine kinase showed a fivefold elevation in single hosts 4 days after implantation; by 14 days its levels were about 200 times above normal and had also risen in the parabiotic hosts (20-fold) and host partners (fourfold). Implantation of fibrosarcoma caused qualitatively similar but generally less pronounced changes in these three enzymes in livers of single hosts, parabiotic hosts, and host partners. The splenic thymidine kinase 14 days after implantation was increased from control levels of about 3 U/g to 50.6, 44.8, and 13.5 U/g in single hosts, parabiotic hosts, and host partners, respectively. At later stages, 17 to 20 days after implantation of the lymphoma, appreciable amounts of thymidine kinase appeared in the plasma: The units of activity per milliliter were 6.2 in single hosts, 0.79 in parabiotic hosts, and 0.55 in host partners (control <0.05). Our observations on the changes in hepatic and splenic enzymes in parabiotic rats suggest that effects of neoplasms on distant host tissues are mediated by humoral factors. The less pronounced responses in parabiotic than in single hosts indicate that the tumor-free partner affords some ''protection'' against these systemic effects.

• Research Organization: Harvard Medical School, Boston
• OSTI ID: 5143555
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 60:4; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
ANIMALS
ARGINASE
BIOLOGICAL EFFECTS
BODY
DIGESTIVE SYSTEM
DISEASES
ENZYMES
FIBROSARCOMAS
GLANDS
HYDROLASES
LIVER
LYMPHOMAS
MAMMALS
METABOLISM
MOSAICISM
NEOPLASMS
ORGANS
PARABIOSIS
PHOSPHOTRANSFERASES
RATS
RODENTS
SARCOMAS
SPLEEN
TRANSFERASES
VERTEBRATES