Cellulase immobilized on poly-L-glutamic acid
Journal Article
·
· Biotechnol. Bioeng.; (United States)
Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum pH and temperature were determined to be 5.5 and 55 degrees C, respectively. The stability of IC against change in the pH and temperature was improved by the immobilization. Solvolysis of 3N-NaOH-treated cellulose, with IC under the optimum conditions found here, led to the production of low-molecular-weight compounds. 15 references.
- Research Organization:
- Kyoto Institute of Technology, Matsugasaki, Japan
- OSTI ID:
- 6564143
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:2; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MECHANICAL STRUCTURES
MOLECULAR WEIGHT
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
PH VALUE
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
STABILITY
SUPPORTS
TEMPERATURE EFFECTS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MECHANICAL STRUCTURES
MOLECULAR WEIGHT
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
PH VALUE
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
STABILITY
SUPPORTS
TEMPERATURE EFFECTS