Preparation and properties of immobilized amyloglucosidase
Journal Article
·
· Biotechnol. Bioeng.; (United States)
Amyloglucosidase was immobilized on a copolymer of methyl methacrylate and 2-di-methylaminoethyl methacrylate. The resulting immobilized amyloglucosidase has 19% of the soluble enzyme specific activity. The pH optimum of immobilized amyloglucosidase is shifted towards acidity by 1.9 units. The temperature optimum of immobilized enzyme is shifted upward by 5 degrees C. The immobilized amyloglucosidase has the maximum stability at pH 4.6, whereas the soluble enzyme has maximum stability at pH 5.5. While soluble amyloglucosidase has a maximum thermal stability at 50 degrees C, the stability of the immobilized amyloglucosidase steadily decreases with the increase in temperature. (Refs. 7).
- Research Organization:
- Polymer Div., Central Leather Research Inst., Adyar, Madras 600 020 India
- OSTI ID:
- 5301772
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:10; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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