GTP-binding peptide of beta-tubulin. Localization by direct photoaffinity labeling and comparison with nucleotide-binding proteins
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6561751
The binding site of the guanine moiety of GTP on beta-tubulin was located within the peptide consisting of residues 63-77, AILVDLEPGTMDSVR. The result was obtained using direct photoaffinity labeling, peptide sequencing, and limited proteolysis. Peptides were identified by end-labeling with a monoclonal antibody against beta-tubulin whose epitope was located between 3 and 4 kDa from the C terminus. The sequence of the GTP-binding site is consistent with predictions from other GTP-binding proteins such as elongation factor Tu or ras p21.
- Research Organization:
- Max-Planck-Unit for Structural Molecular Biology, DESY, Hamburg (Germany, F.R.)
- OSTI ID:
- 6561751
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:29; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Localization of the exchangeable nucleotide binding domain in beta-tubulin
Synthesis and application of a novel GTP phosphonamide photoaffinity reagent: Study of exchangeable GTP-binding domain on tubulin
Identification of the guanosine interacting peptide of the GTP binding site of. beta. -tubulin using 8N/sub 3/GTP
Journal Article
·
Thu Mar 27 23:00:00 EST 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:5531970
Synthesis and application of a novel GTP phosphonamide photoaffinity reagent: Study of exchangeable GTP-binding domain on tubulin
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:5605031
Identification of the guanosine interacting peptide of the GTP binding site of. beta. -tubulin using 8N/sub 3/GTP
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6029263
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ANTIBODIES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CHYMOTRYPSIN
DECOMPOSITION
DOMESTIC ANIMALS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLING
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
PROTEOLYSIS
RECEPTORS
SERINE PROTEINASES
SWINE
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ANTIBODIES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CHYMOTRYPSIN
DECOMPOSITION
DOMESTIC ANIMALS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLING
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
PROTEOLYSIS
RECEPTORS
SERINE PROTEINASES
SWINE
TRACER TECHNIQUES
VERTEBRATES