Identification of the guanosine interacting peptide of the GTP binding site of. beta. -tubulin using 8N/sub 3/GTP
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6029263
It has been shown earlier that 8N/sub 3/GTP is an effective biological mimic of GTP in that it interacts at the exchangeable GTP binding site and causes tubulin to polymerize in a fashion quite similar to GTP. Also, using ..cap alpha.. and ..gamma..(/sup 32/P)8N/sub 3/GTP it is now possible to selectively and effectively photolabel the ..beta..-subunit. A complete sequence of the ..cap alpha.. and ..beta..-subunits of porcine brain tubulin has been reported. They now report on conditions which optimize the photoinsertion of (/sup 32/P)8N/sub 3/GTP selectively into the ..beta..-subunit of tubulin and the use of cation-exchange column chromatography to assist in the purification of the photolabeled peptide. Using two slightly different procedures they have isolated the 8N/sub 3/GTP photolabeled peptide and identified its partial sequence as A-I-L-V-D-L-E-P-G-T which agrees with the highly conserved region of residues 63-72 of ..beta..-tubulin.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 6029263
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
FRACTIONATION
GUANYLIC ACID
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
SEPARATION PROCESSES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
FRACTIONATION
GUANYLIC ACID
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
SEPARATION PROCESSES
TRACER TECHNIQUES