A photoactive phosphonamide derivative of GTP for the identification of the GTP-binding domain in beta-tubulin
Journal Article
·
· Bioconjugate Chemistry; (United States)
- Univ. of Kentucky, Lexington (USA)
A GTP photoaffinity probe (125I-APTG) was developed that incorporated an (125I)-N-(4-azidophenyl)-2-amino-3-(4-hydroxy-3-iodophenyl)propionamide group at the gamma-position of GTP through a phosphonamide linkage. A combination of saturation and GTP protection studies (90% protection at 25 microM GTP with an apparent Kd of 5 microM) validated the use of this new probe as a satisfactory GTP mimic. This probe offered the advantage of possessing an 125I radiolabel external to the GTP moiety, in contrast to the previously reported (gamma 32P)-8-N3GTP that possessed an internal 32P radiolabel. This novel feature accommodated the purification of photolabeled peptides using a combination of ion-exclusion, gel filtration, and HPLC techniques. (125I)APTG was used to identify a peptide (beta:65-79) in the exchangeable GTP-binding domain of the beta-subunit of tubulin.
- OSTI ID:
- 5272289
- Journal Information:
- Bioconjugate Chemistry; (United States), Journal Name: Bioconjugate Chemistry; (United States) Vol. 1:5; ISSN 1043-1802; ISSN BCCHE
- Country of Publication:
- United States
- Language:
- English
Similar Records
Synthesis and application of a novel GTP phosphonamide photoaffinity reagent: Study of exchangeable GTP-binding domain on tubulin
Identification of the guanosine interacting peptide of the GTP binding site of. beta. -tubulin using 8N/sub 3/GTP
Photoaffinity labeling of tubulin subunits with a photoactive analogue of vinblastine
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:5605031
Identification of the guanosine interacting peptide of the GTP binding site of. beta. -tubulin using 8N/sub 3/GTP
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6029263
Photoaffinity labeling of tubulin subunits with a photoactive analogue of vinblastine
Journal Article
·
Mon Jan 12 23:00:00 EST 1987
· Biochemistry; (United States)
·
OSTI ID:6528379
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AZIDES
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
CHEMISTRY
CHROMATOGRAPHY
CPB
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FILTRATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NITROGEN COMPOUNDS
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOTOCHEMISTRY
PROTEINS
RADIOISOTOPES
REAGENTS
RECEPTORS
RESONANCE
SEPARATION PROCESSES
SPECTROPHOTOMETRY
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AZIDES
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
CHEMISTRY
CHROMATOGRAPHY
CPB
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FILTRATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NITROGEN COMPOUNDS
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOTOCHEMISTRY
PROTEINS
RADIOISOTOPES
REAGENTS
RECEPTORS
RESONANCE
SEPARATION PROCESSES
SPECTROPHOTOMETRY
TRACER TECHNIQUES