Localization of the exchangeable nucleotide binding domain in beta-tubulin
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Limited proteolysis of tubulin by alpha-chymotrypsin cleaved the beta-subunit preferentially at Tyr 281, generating primarily 35 kD and 17 kD fragments which were located in the amino terminal and the carboxy terminal regions, respectively. A small amount of a 19 kD fragment from the C-terminal end was also produced. Alpha-Chymotrypsin-treated tubulin retained the ability to exchange GTP and covalently incorporate nucleotide by direct photoaffinity labeling. SDS-PAGE and autoradiography analysis of the (alpha-/sup 32/P) GTP-labeled alpha-CT-treated tubulin showed that the 35 kD fragment was almost exclusively labeled, indicating that the exchangeable GTP binding domain resides in the amino terminal region of the beta-subunit.
- Research Organization:
- Univ. of Kansas, Lawrence
- OSTI ID:
- 5531970
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 3; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHYMOTRYPSIN
CLEAVAGE
COVALENCE
CRYSTAL STRUCTURE
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROSTRUCTURE
MICROTUBULES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERS
PROTEINS
PROTEOLYSIS
RADIOISOTOPES
REACTION KINETICS
SERINE PROTEINASES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHYMOTRYPSIN
CLEAVAGE
COVALENCE
CRYSTAL STRUCTURE
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROSTRUCTURE
MICROTUBULES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERS
PROTEINS
PROTEOLYSIS
RADIOISOTOPES
REACTION KINETICS
SERINE PROTEINASES
TRACER TECHNIQUES