Effect of 'irrelevant' IgG protein selection on target uptake ratios of radiolabeled antibodies

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Invest. Radiol.; (United States)

DOI:https://doi.org/10.1097/00004424-198807000-00011· OSTI ID:6560442

Freimanis, R; Zoghbi, S S; Gottschalk, A

The uptake ratios of radiolabeled antibodies (Ab) commonly are two to three times the uptake ratios of the normal surrounding tissue. In an experimental crush injury model, we also defined a two to three times greater range of uptake of type I anticollagen antibody (Ac-Ab-I) from 1.5 hours to 48 hours after tail injury in the rat. Specific target uptake, however, often is further categorized by comparing the uptake ratio of Ab to that of a nonspecific protein. We present data to indicate that this nonspecific uptake may represent a significant variable. We found that sheep IgG gave consistently less nonspecific uptake in our model than did rabbit IgG. In the acute model (protein injected 1.5 hours after injury), damaged: normal tail ratios were Ac-Ab-I = 2.34, sheep = 0.8, and rabbit = 1.6. The extent of specific Ab uptake seems dependent upon the choice of irrelevant IgG controls.

• Research Organization: Bowman Gray School of Medicine, Winston-Salem, NC (USA)
• OSTI ID: 6560442
• Journal Information: Invest. Radiol.; (United States), Journal Name: Invest. Radiol.; (United States) Vol. 23:7; ISSN INVRA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BODY FLUIDS
COLLAGEN
DOMESTIC ANIMALS
ERYTHROCYTES
GLOBULINS
IMMUNOGLOBULINS
ISOTOPES
LABELLING
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
RABBITS
RADIOISOTOPES
RATS
RODENTS
RUMINANTS
SCLEROPROTEINS
SHEEP
UPTAKE
VERTEBRATES