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Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme

Journal Article · · Journal of Nuclear Medicine; (USA)
OSTI ID:5960009
; ; ; ; ; ;  [1]
  1. USSR Cardiology Research Center, Moscow (USSR)
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The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in non-edematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91% of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17% of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44% versus 0.89% in the control.
OSTI ID:
5960009
Journal Information:
Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 32:3; ISSN 0161-5505; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BIOLOGICAL EFFECTS
BODY
DISTRIBUTION
EDEMA
ENZYME ACTIVITY
ENZYMES
GLOBULINS
HYDROLASES
IMMUNOGLOBULINS
INDIUM ISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
MAMMALS
MATERIALS
MICE
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PROTEINS
RATS
RESPIRATORY SYSTEM
RODENTS
SYMPTOMS
TISSUE DISTRIBUTION
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES