Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)

OSTI ID:6009849

Danilov, S M; Muzykantov, V R; Martynov, A V; Atochina, E N; Sakharov, I Yu; Trakht, I N; Smirnov, V N ^[1]

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1. Institute of Experimental Cardiology, Moscow (USSR)

125I-labeled mouse monoclonal antibody (MoAb) to human angiotensin-converting enzyme (ACE), termed 9B9 and cross-reacting with rat and monkey ACE, when injected into the circulation, accumulates in the lung in up to 10 to 20 greater concentrations than in other organs and blood. That 111In-labeled MoAb 9B9 also accumulates in the lungs of both rats and monkeys very selectively, was clearly revealed by gamma-scintigraphy. Unlike polyclonal anti-ACE antibodies that induce an immunodependent lethal reaction when administered intravenously, MoAb 9B9 was well tolerated by rats even at very high doses (up to 300 mg/kg/body weight). At the same time, the administration of this antibody (which does not inhibit the catalytic activity of ACE) resulted in both a 3-fold decrease of the lung ACE activity and an increase in the activity of serum ACE. The highly organ-specific, nondamaging accumulation of the MoAb 9B9 makes it a promising vector for targeted drug delivery to the lung, for modeling of lung pathology, and for gamma-scintigraphic visualization of the lung vascular bed. We also suggest that MoAb 9B9 accumulation in the lung may serve as a highly sensitive marker of lung vessel damage upon various lung pathology.

• OSTI ID: 6009849
• Journal Information: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA), Journal Name: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA) Vol. 64:1; ISSN 0023-6837; ISSN LAINA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
MAMMALS
MAN
MICE
MONKEYS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
PRIMATES
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RATS
RESPIRATORY SYSTEM
RODENTS
SCINTISCANNING
SPECIFICITY
TRACER TECHNIQUES
VERTEBRATES