Mapping of the versican proteoglycan gene (CSPG2) to the long arm of human chromosome 5 (5q12-5q14)

Journal Article · Tue Dec 01 04:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80103-X· OSTI ID:6553858

Iozzo, R V; Naso, M F; Cannizzaro, L A ^[1]; Wasmuth, J J; McPerson, J D ^[2]

---

1. Thomas Jefferson Univ., Philadelphia, PA (United States)
2. Univ. of California, Irvine, CA (United States)

Versican is a major chondroitin sulfate proteoglycan of vascularized connective tissues whose eponym reflects its functional versatility in macromolecular affinity and interactions. In this report the authors have localized the versican gene (CSPG2) to the long arm of human chromosome 5 by utilizing a combination of somatic cell hybrids, Southern blotting, polymerase chain reaction, and chromosomal in situ hybridization. The proteoglycan gene segregated concordantly with hybrid cell lines containing the long arm of chromosome 5, comprising the 5q12-q14 band regions. To refine this locus further, they screened a chromosome 5-specific library and isolated several genomic clones encoding a portion of the 5[prime] end of versican. One of these genomic clones was used as a probe for in situ hybridization of human chromosome metaphases. The results corroborated the data obtained using somatic cell hybrids and further refined the assignment of the versican gene to the narrow band region of 5q12-5q14, with the primary site likely to be 5q13.2. The availability of novel genomic clones and the mapping data presented here will make possible the identification of any defect genetically linked to this proteoglycan gene. 27 refs., 4 figs.

• OSTI ID: 6553858
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 14:4; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA-CLONING
GENES
GENETIC ENGINEERING
GENETIC MAPPING
GLYCOPROTEINS
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
PROTEINS