Chromosome translocation frequencies measured in interphase lymphocytes of A-bomb survivors

Conference · Fri Jan 01 04:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6552170

Cantu, A; Richards, C; Burroughs, F; Lucas, J N ^[1]

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1. Lawrence Livermore National Lab., CA (United States)

The authors have compared metaphase chromosome translocation frequencies with interphase chromosome aberration frequencies measured in lymphocytes of A-bomb survivors using FISH. Whole chromosome probes for chromosomes 1, 2, and 4 were used to measure metaphase translocations, and a repetitive probe that binds to the paracentromeric 1q12 locus of chromosome 1 (pUC1.77) was used to measure interphase chromosome aberrations. Doses for the individuals studied ranged from 0 to 4 Gy. The interphase hybridization pattern appeared as one or two distinct spots in normal interphase cells, and as three distinct spots in cells carrying a translocation. Although pUC1.77 is small ([approximately]1% of genome), the increased speed in scoring interphase cells (a factor of 50 faster than scoring metaphase cells) compensates for the small size of the probe. A linear correlation was found between interphase and metaphase aberration frequencies. The data suggest that aberration frequencies may be obtained from interphase cells of individuals exposed decades previously, where dicentrics have decayed. Detection of interphase chromosome aberrations should facilitate automation of chromosome aberration scoring.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6552170
• Report Number(s): CONF-9303114--
• Conference Information: Journal Name: Cytometry (Baltimore); (United States) Journal Volume: 6
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560151* -- Radiation Effects on Animals-- Man
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
A-BOMB SURVIVORS
ANIMAL CELLS
AUTOMATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DETECTION
DNA HYBRIDIZATION
DOSES
FLUORESCENCE
HUMAN CHROMOSOME 2
HUMAN CHROMOSOMES
HUMAN POPULATIONS
HYBRIDIZATION
LEUKOCYTES
LUMINESCENCE
LYMPHOCYTES
MATERIALS
MUTATIONS
POPULATIONS
RADIATION DOSES
SOMATIC CELLS