Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Cytometry (Baltimore); (USA)

DOI:https://doi.org/10.1002/cyto.990110113· OSTI ID:7136747

Cremer, T; Popp, S; Emmerich, P; Lichter, P; Cremer, C ^[1]

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1. Institut fuer Humangenetik und Anthropolgie, Heidelberg (Germany, F.R.)

Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes in irradiated human peripheral lymphocytes. In addition, probe pUC1.77 was used to mark the 1q12 subregion in normal and aberrant chromosomes 1. Low LET radiation (60Co-gamma-rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreads were obtained. Aberrations of the specifically stained chromosomes, such as deletions, dicentrics, and rings, were readily scored after in situ hybridization with either the 1q12 specific probe or DNA-library inserts. By the latter approach, translocations of the specifically stained chromosomes could also be reliably assessed. A linear increase of the percentage of specifically stained aberrant chromosomes was observed when plotted as a function of the square of the dose D. A particular advantage of this new approach is provided by the possibility to delineate numerical and structural chromosome aberrations directly in interphase nuclei. These results indicate that cytogenetic monitoring of ionizing radiation may be considerably facilitated by CISS-hybridization.

• OSTI ID: 7136747
• Journal Information: Cytometry (Baltimore); (USA), Vol. 11:1; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
LYMPHOCYTES
GENETIC RADIATION EFFECTS
CHROMOSOMES
COBALT 60
DNA HYBRIDIZATION
DOSE-RESPONSE RELATIONSHIPS
FLUORESCENCE
GAMMA RADIATION
LET
MAN
MITOSIS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENERGY TRANSFER
GENETIC EFFECTS
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LUMINESCENCE
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOT
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture