Biological dosimetry by interphase chromosome painting

Journal Article · Mon Jan 01 04:00:00 EST 1996 · Radiation Research

DOI:https://doi.org/10.2307/3579195· OSTI ID:478298

Durante, M; George, K; Yang, T C ^[1]

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1. Lyndon B. Johnson Space Center, Houston, TX (United States)

Both fluorescence in situ hybridization of metaphase spreads with whole-chromosome probes and premature chromosome condensation in interphase nuclei have been used in the past to estimate the radiation dose to lymphocytes. We combined these techniques to evaluate the feasibility of using painted interphase chromosomes for biodosimetry. Human peripheral lymphocytes were exposed to {gamma} rays and fused to mitotic Chinese hamster cells either immediately after irradiation or after 8 h incubation at 37{degrees}C. Interphase or metaphase human chromosomes were hybridized with a composite probe specific for human chromosomes 3 and 4. The dose-response curve for fragment induction immediately after irradiation was linear; these results reflected breakage frequency in the total genome in terms of DNA content per chromosome. At 8 h after irradiation, the dose-response curve for chromosome interchanges, the prevalent aberration in interphase chromosomes, was linear-quadratic and similar to that observed for metaphase chromosomes. These results suggest that painting prematurely condensed chromosomes can be useful for biological dosimetry when blood samples are available shortly after the exposure, or when interphase cells are to be scored instead of mitotic cells. 32 refs., 7 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 478298
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 145; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL RADIATION EFFECTS
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
DELAYED RADIATION EFFECTS
DNA HYBRIDIZATION
DOSE-RESPONSE RELATIONSHIPS
DOSIMETRY
EFFICIENCY
EVALUATION
FLUORESCENCE
GAMMA RADIATION
GENETIC RADIATION EFFECTS
GENETICALLY SIGNIFICANT DOSE
GENOME MUTATIONS
HUMAN CHROMOSOMES
LYMPHOCYTES
MITOSIS
MUTATION FREQUENCY
PROBES
RADIATION DOSES