Mechanism of stimulation of antibody-forming ability of bone marrow cells of mice immunized with staphylococci

Journal Article · Wed Dec 31 23:00:00 EST 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:6550060

Lyashchenko, K P; Golovanova, T A; Bobrovnik, S A

The purpose of this paper is to study the formation of the ability of the bone marrow cells of mice immunized with staphylococci to create antibodies to this antigen. The research includes a study of the effect of the irradiation in vitro of the bone marrow cells on their stimulating activity and the role played by the thymus and spleen in the formation of this activity. Experiments were carried out on CBA and BALB/c mice as well as on mice with congenital absence of the thymus. The bone marrow cell donors were immunized intravenously with staphylococcal corpuscular antigen. Receptor mice were irradiated with cobalt 60 gamma radiation and injected intravenously with bone marrow cell extract from the immunized donors and were immunized with the antigen. Spleen cells were labelled with chromium 51 and injected intravenously into intact syngeneic recipients together with as well as without the antigen. Three days later the level of radioactivity in the spleen and femora of the animals was determined by scintillation counting. Total radioactivity of the bone marrow was calculated. Irradiation of the bone marrow cells of immunized animals was shown to abolish their stimulating effect on the humoral immune response of intact syngeneic recipients to the staphylococcal corpuscular antigen. Consequently, the immunostimulating effect of bone marrow cells is realized through the proliferating and radiosensitive lymphoid cells rather than through the macrophages.

• Research Organization: T.G. Shevchenko Kiev Univ., USSR
• OSTI ID: 6550060
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 102:8; ISSN BEXBA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
EXTERNAL IRRADIATION
IMMUNE REACTIONS
IMMUNITY
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INTAKE
INTRAVENOUS INJECTION
IRRADIATION
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEDICINE
MICE
MICROORGANISMS
ORGANS
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOSENSITIVITY
RODENTS
SCINTILLATION COUNTING
SOMATIC CELLS
SPLEEN
SPLEEN CELLS
SPLENECTOMY
STAPHYLOCOCCUS
STIMULATION
SURGERY
SYNTHESIS
THYMUS
TRACER TECHNIQUES
VERTEBRATES