Influence of chemical effectors of reactive oxygen species and GSH depletion on cell survival following photodynamic treatment and ionizing radiation
The influence of cellular glutathione (GSH) levels on the response to photodynamic treatment (PDT) in vitro was determined in cells which were depleted of GSH by buthionine sulfoximine (BSO), or which were genetically GSH deficient. The effects of GSH depletion on cellular radiosensitivity were studied in parallel for comparison. BSO treatment which reduced GSH levels in four cell lines (CHO, V-79, EMT6, RIF) to approximately 80% and 30% of controls, or to undetectable levels, uniformly decreased cell survival. This decrease was directly related to GSH depletion. GSH level-dependent aerobic radiosensitization following BSO treatment was found in all four cell lines, and cell survival changes were expressed in the survival curves. Cell survival of GSH deficient human fibroblasts (GM 3877) was decreased following PDT and gamma irradiation when compared to their normal counterparts (GM 5659). Neither BSO nor GSH interfered with cellular porphyrin uptake or singlet oxygen production during the photodynamic process.
- Research Organization:
- State Univ. of New York, Buffalo (USA)
- OSTI ID:
- 6545506
- Country of Publication:
- United States
- Language:
- English
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Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
CHO CELLS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
GLUTATHIONE
HAMSTERS
IONIZING RADIATIONS
LUNGS
MAMMALS
MAN
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
VERTEBRATES