Action of DTPA on hepatic plutonium. II. DTPA-induced removal of monomeric plutonium from mouse liver parenchymal cells

Journal Article · Sun Oct 01 04:00:00 EDT 1978 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574937· OSTI ID:6540058

Bhattacharyya, M H; Peterson, D P; Lindenbaum, A

Liver parenchymal cells were isolated 6 and 24 hr following the administration of diethylenetriaminepentaacetic acid (DTPA, 0.25 mmole/kg) to mice previously injected with /sup 239/Pu-citrate (4.4 ..mu..Ci/kg). Isolated parenchymal cells contained 440 dpm Pu/10/sup 6/ cells at 24 hr after Pu injection, just prior to DTPA administration. The PU content decreased to 330 dpm/10/sup 6/ cells at 6 hr and 140 dpm/10/sup 6/ cells at 24 hr after DTPA administration. Thus DTPA induced a striking decrease in the Pu content of isolated liver parenchymal cells. Parenchymal cells isolated from control mice not treated with DTPA changed little in Pu content from 24 to 48 hr after Pu injection. By 24 hr after DTPA treatment, the decrease in the Pu content of isolated liver parenchymal cells could account for the DTPA-induced release of Pu from the intact liver. Thus in the liver DTPA appears to act preferentially on the Pu associated with parenchymal cells. Liver parenchymal cells isolated 6 hr after DTPA administration and containing 330 dpm Pu/10/sup 6/ cells were incubated in vitro in the absence of added DTPA. After 18 hr of incubation the cells contained 130 dpm Pu/10/sup 6/ cells. This level corresponds to the level observed in cells isolated 24 hr after DTPA administration. Cells isolated from untreated mice lost only 15% of their Pu content during a similar in vitro incubation. Thus, by 6 hr after DTPA administration to the mouse, isolated liver parenchymal cells appeared to retain their ability to release Pu in vitro with no need for additional exposure to DTPA. The physiological significance of this finding is discussed.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 6540058
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 76:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE COMPOUNDS
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHELATING AGENTS
CITRATES
CLEARANCE
DIGESTIVE SYSTEM
DISPERSIONS
DRUGS
DTPA
EVEN-ODD NUCLEI
EXCRETION
GLANDS
HEAVY NUCLEI
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPES
LIVER
MAMMALS
MICE
MIXTURES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLUTONIUM 239
PLUTONIUM COMPOUNDS
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPROTECTIVE SUBSTANCES
RESPONSE MODIFYING FACTORS
RETENTION
RODENTS
SOLUTIONS
SOMATIC CELLS
TRANSURANIUM COMPOUNDS
VERTEBRATES
YEARS LIVING RADIOISOTOPES