Action of DTPA on hepatic plutonium. III. Evidence for a direct chelation mechanism for DTPA-induced excretion of monomeric plutonium into rat bile. [/sup 239/Pu]

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575119· OSTI ID:5673887

Bhattacharyya, M H; Peterson, D P

Trisodium calcium diethylenetriaminepenta(2-/sup 14/C)acetic acid ((/sup 14/C)DTPA, 0.25 nmole/kg, 0.210 mCi/mmole) was administered via the jugular vein to three rats whose bile ducts and urinary bladders were cannulated. Bile and urine were collected for 24 h after injection, and tissues and excreta were then analyzed for /sup 14/C content. (/sup 14/C)DTPA was identified in the bile samples by cochromatography with unlabeled DTPA on an anion-exchange column system. Data from this experiment demonstrated the existence of a minor excretion pathway for DTPA into rat bile, accounting for 0.12% of the injected dose by 24 h after administration. Bile was collected for 24 h following the administration of unlabeled Na/sub 3/(CaDTPA) (0.25 mmole/kg, iv) to a rat which had received /sup 239/Pu(IV)-citrate (5.7 ..mu..Ci/kg, iv) 24 h prior to DTPA. Bile samples containing Pu were analyzed using anion-exchange column chromatography. Eighty to ninety percent of the Pu present in bile chromatographed as a single peak in the position of the Pu-DTPA complex. Cochromatograhy of the 0- to 6-h bile sample with a /sup 239/Pu-(/sup 14/C)DTPA complex revealed that the Pu appearing in bile following DTPA treatment comigrated with the /sup 239/Pu-(/sup 14/C)DTPA complex. These data provide strong evidence that, in the rat, DTPA acts to remove hepatic deposits of monomeric Pu by formation of the Pu-DTPA complex followed by excretion of the complex into bile.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5673887
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 80:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE COMPLEXES
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
AMINO ACIDS
ANIMALS
BILE
BILIARY TRACT
BIOLOGICAL EFFECTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLADDER
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMATOGRAPHY
CLEARANCE
COMPLEXES
DATA
DATA FORMS
DIGESTIVE SYSTEM
DRUGS
DTPA
EVEN-ODD NUCLEI
EXCRETION
EXPERIMENTAL DATA
GLANDS
HEAVY NUCLEI
INFORMATION
INJECTION
INTAKE
INTRAVENOUS INJECTION
ION EXCHANGE CHROMATOGRAPHY
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
MAMMALS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLUTONIUM 239
PLUTONIUM COMPLEXES
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RADIOPROTECTIVE SUBSTANCES
RATS
RENAL CLEARANCE
RESPONSE MODIFYING FACTORS
RETENTION FUNCTIONS
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSURANIUM COMPLEXES
URINARY TRACT
VERTEBRATES
YEARS LIVING RADIOISOTOPES