Biochemical response of squirrel monkeys to ozone. [Biochemical changes in blood and lung tissue following acute inhalation]

Journal Article · Fri Sep 01 04:00:00 EDT 1978 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287397809529696· OSTI ID:6537963

Clark, K W; Posin, C I; Buckley, R D

Biochemical studies were performed on blood and lung tissue of squirrel monkeys (Saimiri sciureus) following acute exposure to 0.75 ppM ozone (O/sub 3/) for 4 h/d for 4 consecutive days. One group of animals was sacrificed at the end of the last exposure day and another group was sacrificed 4 d later after the last exposure. Evidence was sought for oxidation-induced changes known to occur in rodents when high levels of O/sub 3/ are inhaled. A significant increase in red blood cell membrane fragility was observed, as well as significant decreases in red blood cell glutathione and erythrocyte acetylcholinesterase; however, the red blood cell enzymes, lactic acid dehydrogenase (LDH), and glucose-6-phosphate dehydrogenase (G6PDH) were not changed significantly. Lung tissue analysis showed that lipid peroxidation was markedly increased and tissue vitamin E levels were significantly decreased. The tissue enzymes G6PDH, glutathione reductase, and LDH significantly increased in activity. No significant changes were seen in either superoxide dismutase of malic acid dehydrogenase. The results of this experiment indicate that O/sub 3/, or reaction products resulting from O/sub 3/-tissue interaction in the lung, pass the air-blood barrier and are capable of producing biochemical changes in blood as well as in lung tissue.

• Research Organization: Rancho Los Amigos Hospital, Downey, CA
• OSTI ID: 6537963
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 4:5-6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550900 -- Pathology
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHOLINESTERASE
CLEARANCE
DEHYDROGENASES
DISEASES
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
ESTERASES
EXCRETION
GLUTATHIONE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INHALATION
INTAKE
LACTATE DEHYDROGENASE
LUNG CLEARANCE
LUNGS
MAMMALS
MEMBRANES
MONKEYS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OZONE
PATHOLOGICAL CHANGES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PORPHYRINS
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
VERTEBRATES
VITAMIN E
VITAMINS