Spleen colonies from peritoneal cell-coated membranes seeded with bone marrow and inserted into the peritoneum of lethally irradiated mice
When cellulose acetate disks are placed in the peritoneal cavity of mice they become coated with cells, primarily macrophages. Colonies of granulocytes are produced on CAM when CAM-bearing mice are injected ip with syngeneic bone marrow. The cell which forms these colonies has many features in common with cells which form colonies of neutrophils and macrophages in semisolid media. CFU-ML and CFU-NM are thought to be descendants of pluripotent stem cells such as those which are assayed by the formation of spleen colonies in lethally irradiated mice. The present studies were undertaken to determine if CFU-S were present on CAM. Disks were inserted ip and left for 7 days; the mouse was then injected with marrow, and after 1 or 2 days CAM were transferred to a lethally irradiated mouse. Alternately, CAM were removed incubated in vitro with marrow for 1 h, placed in a normal mouse, and after 1 or 2 days transferred to a lethally irradiated mouse. Spleen colonies and other measures of splenic hematopoiesis were made 8 to 15 days after CAM transfer, or recipients were observed for 30-day survival after CAM transfer. CFU-S were present after either in vivo or in vitro seeding. In vitro seeding of CAM with 1.12 x 10/sup 7/ cells in 0.4 ml of HBSS resulted in 7.8 +- 1.6 spleen colonies at 8 days. By 15 days, colonies were confluent and spleens enlarged. Percentage /sup 59/Fe uptake at Day 8, 10, and 15 after CAM insertion also indicated vigorous hematopoiesis. The 30-day survival of lethally irradiated mice with CAM seeded in vitro was 15/40 compared to 2/20 for recipients of unseeded CAM. These data suggest the presence of pluripotent stem cells on CAM but do not indicate whether or not CAM colonies are derived from such cells.
- Research Organization:
- Univ. of Pittsburgh, PA
- OSTI ID:
- 6537945
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 85:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW
CARBOHYDRATES
CELLULOSE
CESIUM 137
CESIUM ISOTOPES
CFU
COLONY FORMATION
CONNECTIVE TISSUE CELLS
ERYTHROPOIESIS
EVEN-ODD NUCLEI
EXTERNAL IRRADIATION
HEMATOPOIETIC SYSTEM
IMPLANTS
INTERMEDIATE MASS NUCLEI
IRON 53
IRON ISOTOPES
IRRADIATION
ISOTOPES
LETHAL IRRADIATION
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
POLYSACCHARIDES
RADIATION EFFECTS
RADIOISOTOPES
RADIOSENSITIVITY
RESPONSE MODIFYING FACTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN COLONY FORMATION
STEM CELLS
SURVIVAL CURVES
TISSUES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES