Degradation of cadmium-thionein in rat liver and kidney
Journal Article
·
· J. Toxicol. Environ. Health; (United States)
(/sup 3/H)Cystine and /sup 115m/Cd were incorporated into hepatic and renal Cd-thionein in response to sc administration of 4.4 ..mu..mol of Cd/sup 2 +/ containing /sup 115m/Cd. Cd-thionein-bound /sup 115m/Cd reached a plateau by 24 and 72 h after the Cd/sup 2 +/ injection in liver and kidney, respectively. The half-life (t/sub 1/2/) of /sup 3/H-labeled hepatic Cd-thionein was 3.5 d, whereas the average t/sub 1/2/ of the soluble proteins was 3.7d. The t/sub 1/2/ of /sup 3/H-labeled renal Cd-thionein was 3.7d, whereas the average t/sub 1/2/ of the renal proteins was 3.8 d. In marked contrast, the /sup 115m/Cd content of both hepatic and renal Cd-thionein was virtually unchanged, even 9 d after administration of this radionuclide. These data indicate that the protein moiety of metallothionein is degraded, although there appears to be a concomitant rebinding of Cd/sup 2 +/ to nascent thionein polypeptide chains. Thus the lack of metallothionein degradation per se does not account for the long-term retention of Cd/sup 2 +/ in liver and kdney during chronic exposure.
- Research Organization:
- Rutgers Univ., New Brunswick, NJ
- OSTI ID:
- 6537884
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 4:5-6; ISSN JTEHD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CADMIUM 115
CADMIUM COMPLEXES
CADMIUM ISOTOPES
CARBOXYLIC ACIDS
CATABOLISM
COMPLEXES
CYSTINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISULFIDES
EVEN-ODD NUCLEI
GLANDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SUBCUTANEOUS INJECTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CADMIUM 115
CADMIUM COMPLEXES
CADMIUM ISOTOPES
CARBOXYLIC ACIDS
CATABOLISM
COMPLEXES
CYSTINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISULFIDES
EVEN-ODD NUCLEI
GLANDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SUBCUTANEOUS INJECTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES