Reguation of induced thionein gene expression in cultured mammalian cells: effects of protein synthesis inhibition on translatable thionein mRNA levels in regulatory variants of the CHO cell
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The effects of protein synthesis inhibitors, cycloheximide and puromycin, on functional thionein mRNA levels have been examined to further delineate the regulation of thionein gene expression in Cd/sup 2 +/-resistant CHO sublines proficient in Cd/sup 2 +/- or Zn/sup 2 +/-stimulated synthesis of metallothioneins. Inhibition of protein synthesis at or shortly before the time of metal-mediated induction only slightly reduces the usual increase in thionein mRNA. Protein synthesis inhibition alone does not significantly increase thionein mRNA levels. However, addition of cycloheximide 4 h after Zn/sup 2 +/ induction increases thionein mRNA activity measured 8 h after induction by at least 40%, suggesting that protein synthesis is required for normal turnover of thionein mRNA.
- Research Organization:
- Los Alamos Scientific Lab., NM
- OSTI ID:
- 6868529
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 93:2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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·
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Related Subjects
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CADMIUM
CYCLOHEXIMIDE
DRUGS
ELEMENTS
FUNGICIDES
HAMSTERS
INHIBITION
MAMMALS
MESSENGER-RNA
METALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PESTICIDES
PROTEINS
PUROMYCIN
RNA
RODENTS
SIDE EFFECTS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES
ZINC
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CADMIUM
CYCLOHEXIMIDE
DRUGS
ELEMENTS
FUNGICIDES
HAMSTERS
INHIBITION
MAMMALS
MESSENGER-RNA
METALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PESTICIDES
PROTEINS
PUROMYCIN
RNA
RODENTS
SIDE EFFECTS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES
ZINC