Aldosterone-reversible decrease in the density of renal peripheral benzodiazepine receptors in the rat after adrenalectomy

Journal Article · Sun Mar 01 04:00:00 EST 1987 · J. Pharmacol. Exp. Ther.; (United States)

OSTI ID:6526180

Basile, A S; Ostrowski, N L; Skolnick, P

A statistically significant decrease in the density of peripheral benzodiazepine receptors was observed in renal membranes of rats beginning 2 weeks after adrenalectomy when compared with sham-operated controls. This decrease in peripheral benzodiazepine receptor density was manifest as a decrease in the maximum binding of two ligands, (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195, without accompanying changes in their Kd for this site. Similar changes were not seen in another aldosterone-sensitive organ, the submandibular salivary gland. The decrease in peripheral benzodiazepine receptor density observed in adrenalectomized rat renal membranes was restored to control levels after 1 week of aldosterone administration using a dose (12.5 micrograms/kg/day) that had no effect on peripheral benzodiazepine receptor density in sham-operated animals. In contrast, dexamethasone administration (50 micrograms/kg/day, 1 week) had no effect on renal peripheral benzodiazepine receptor density when administered to either adrenalectomized or sham-operated rats. Further, adrenal demedullation had no effect on renal peripheral benzodiazepine receptor density or affinity. The decrease in peripheral benzodiazepine receptor density was localized to the renal cortex and the outer stripe of the medulla by gross dissection of renal slices and renal tissue section autoradiography. The specific effect of adrenalectomy on renal peripheral benzodiazepine receptor density, the lack of direct effect of aldosterone on (/sup 3/H) Ro 5-4864 binding and the localization of the change in peripheral benzodiazepine receptor density to the renal cortex and outer stripe suggest that these changes may reflect an adaptation of the renal nephron (possibly the distal convoluted tubule, intermediate tubule and/or the collecting duct) to the loss of mineralocorticoid hormones.

• Research Organization: National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD
• OSTI ID: 6526180
• Journal Information: J. Pharmacol. Exp. Ther.; (United States), Journal Name: J. Pharmacol. Exp. Ther.; (United States) Vol. 3; ISSN JPETA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALECTOMY
ALDEHYDES
ALDOSTERONE
ANIMALS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
CORTICOSTEROIDS
DENSITY
DEXAMETHASONE
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEDICINE
MEMBRANE PROTEINS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PHYSICAL PROPERTIES
PREGNANES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
SURGERY
TRITIUM COMPOUNDS
VERTEBRATES