Autoradiographic localization of benzodiazepine receptors in the rat kidney
The localization of benzodiazepine (BZD) receptors in the rat kidney was studied by autoradiography after in vitro labeling of kidney slices with flunitrazepam. The affinity, density, and rank order of displacement of (/sup 3/H)-flunitrazepam by several BZDs (RO 5-4864 > diazepam > clonazepam) demonstrated that binding was to BZD receptors of the peripheral type. In autoradiograms obtained with tritium-sensitive film, a high density of silver grains was obtained in the outer medulla, with lower densities in the cortex. Binding was absent from the inner medulla (papilla). In higher resolution autoradiograms obtained with an emulsion-coated cover slip procedure, silver grains were seen to be concentrated over a tubular element in both outer medulla and cortex, identifiable by morphology and distribution as the thick ascending limb of the loop of Henle and the distal convoluted tubule. The identity of the labeled tubules was confirmed by immunofluorescent localization in adjacent slices of Tamm-Horsfall protein, a specific marker for these segments of tubules. Investigation of the effects of peripherally specific BZDs such as RO 5-4864 on distal tubule function is indicated.
- Research Organization:
- Univ. of California, San Diego
- OSTI ID:
- 5757314
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 247:5; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CENTRAL NERVOUS SYSTEM AGENTS
CHEMICAL BONDS
DENSITY
DRUGS
FLUORESCENCE
HYDROGEN ISOTOPES
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PHYSICAL PROPERTIES
PSYCHOTROPIC DRUGS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
TRANQUILIZERS
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES