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Differential effect of detergents on (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195 binding to peripheral-type benzodiazepine-binding sites

Journal Article · · Life Sci.; (United States)
;

The present study demonstrates a differential effect of various detergent treatments on (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195 binding to peripheral benzodiazepine binding sites (PBS). Triton X-100 caused a decrease of about 70% in (/sup 3/H)Ro 5-4864 binding to membranes from various peripheral tissues of rat, but had only a negligible effect on (/sup 3/H)PK 11195 binding. A similar effect of Triton X-100 was observed on guinea pig and rabbit kidney membranes. The decrease in (/sup 3/H)Ro 5-4864 binding after treatment with Triton X-100 was apparently due to a decrease in the density of PBS, since the affinity remained unaltered. The detergents 3-((3-cholamidopropyl)-dimethylammonio)-1-propane sulfonate (CHAPS), Tween 20, deoxycholic acid, or digitonin (0.0125%) caused only a minor change in (/sup 3/H)Ro 5-4864 and (/sup 3/H)PK 11195 binding to rat kidney membranes; but when concentrations were substantially increased (0.1%), all detergents caused a decrease of at least 50% in (/sup 3/H)Ro 5-4864 binding, while (/sup 3/H)PK 11195 binding to rat kidney membranes remained unaffected by the first three detergents, with only a minor decrease (15%) after treatment with digitonin.

Research Organization:
Technion-Israel Institute of Technology, Haifa
OSTI ID:
7191850
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:2; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM AGENTS
DETERGENTS
DRUGS
EMULSIFIERS
GUINEA PIGS
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
PSYCHOTROPIC DRUGS
RABBITS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SURFACTANTS
TRACER TECHNIQUES
TRANQUILIZERS
TRITIUM COMPOUNDS
VERTEBRATES
WETTING AGENTS